Base Composition Differences between Avian Myeloblastosis Virus Transfer RNA and Transfer RNA Isolated from Host Cells
نویسندگان
چکیده
منابع مشابه
Base composition differences between avian myeloblastosis virus transfer RNA and transfer RNA isolated from host cells.
Using a novel chemical tritium derivative method, we have determined the base composition of 4S RNA isolated from an RNA tumor virus, the avian myeloblastosis virus, and from normal and neoplastic host cells. Extensive differences were detected, particularly with respect to the amount of methylated bases in the viral RNA. The viral 4S RNA, which fulfills the criteria for designation as transfer...
متن کاملSpecific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase).
The ability of tryptophan tRNA (tRNATrp) to initiate reverse transcription of the 70S RNA of avian RNA tumor viruses suggested that the reverse transcriptase (RNA-dependent DNA polymerase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) might have a specific binding site for the tRNA. A complex of tRNATrp and the avian myeloblastosis virus reverse transcriptase has bee...
متن کاملMinor base composition of "70S-associated" 4S RNA from avian myeloblastosis virus.
Using a chemical isotope derivative method, we have determined the minor base composition of the "70S-associated" 4S RNA isolated from avian myeloblastosis virus. The minor base composition of this "70S-associated" 4S RNA is strikingly similar to that of the corresponding "free" 4S RNA of the virion. This minor base content, plus the capacity to esterify amino acids, establishes that both of th...
متن کاملDifferences between mitochondrial and cytoplasmic transfer RNA and aminoacyl transfer RNA synthetases from rat liver.
The mitochondria of yeast,1 Neurospora,2 and Tetrahymena3 have been shown to contain transfer RNA (tRNA). Barnett et al.4 have found, in addition, that Neurospora mitochondria contain 15 aminoacyl-tRNA synthetases, at least three of which acylate only mitochondrial tRNA. The purpose of this communication is twofold. First we will present evidence showing that, as with eukaryotic microbial syste...
متن کاملHigh molecular weight RNA from avian myeloblastosis virus.
Avian myeloblastosis virus produced in vitro by myeloblastic cells in suspension was labeled with 32P and 14Camino acids. After partial purification of the virus, and pronase digestion, an RNA was extracted by phenol into the aqueous phase. Glycerol gradient centrifugation showed that the 32P-labeled RNA con tained 2 distinct components: a high molecular weight fraction sedimenting at about 67 ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1971
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.68.12.3233